AR37 - Grazing Animals

Animal health and performance responses to grazing ryegrass infected with AR37 have been measured in weaned lambs and hoggets over 10 years at AgResearch Lincoln. Results from the first 6 years were published in 2005 (see paper). These results are updated to 2008 in the tables below.

Sheep growth rates over summer and autumn, when endophyte toxins are likely to be highest, have been better on AR37 than on ryegrass infected with the Standard endophyte. Growth rates on AR37 are similar to the same ryegrass without endophyte or with the AR1 endophyte (see table 1).

Epoxy-janthitrems which are produced by ryegrass infected with AR37, are tremorgenic and can cause ryegrass staggers. Ryegrass staggers is a neuro-muscular disorder which is scored on a 0-5 ascending scale, with 5 being very serious staggers (see table 2). It should noted that there is considerable variation between animals in their susceptibility to ryegrass staggers.

The incidence of ryegrass staggers on pure ryegrass, with AR37, at Lincoln, is less frequent and usually only half as severe as on ryegrass with its Standard endophyte. However AR37 can cause very serious staggers, but in the trials at Lincoln this has occurred in only one year in ten (see table 3).

In more stressful environments (hot drought-prone regions), serious outbreaks may be more frequent. The impact of ryegrass with AR37 on dairy production is currently being assessed in a three year grazing trial but the data have not yet been fully published.

Apart from ryegrass staggers, animals grazing ryegrass with AR37 show none of the adverse responses often associated with grazing ryegrass with Standard endophyte, such as increased body temperatures and respiration rates, more dags and flystrike and lower plasma prolactin concentrations.


TABLE 1: Effect of endophyte, in a range of ryegrass cultivars (pure stands, no clover),
on summer/autumn growth rate (g/day) in grazing lambs
  Endophyte
  Standard AR1 or endophyte-free AR37
  g head-1 day-1
Mean of 6 years 1999 - 2004
Includes Nui ryegrass and an experimental line of ryegrass with AR37
44* 129 131
Mean of 4 years 2005 - 2008
Includes range of commercial cultivars with AR37
98* 137 161
Overall Mean 10 years 71* 133 146
* Growth rates on Standard endophyte are statistically lower than AR37, AR1 or endophyte-free

TABLE 2: Ryegrass staggers scoring criteria: after being driven briskly over 300m
0 No symptoms.
1 Slight muscle spasms around neck, shoulders and flank.
2 Severe muscle spasms and shaking of the head after stress but no loss of co-ordination.
3 As in 2, but with lack of co-ordination when moving.
4 Muscle tremors beginning early in the run (<30 m), soon followed by lack of co-ordination resulting in staggering gait which progresses to the animal falling down. The animal regains its footing after a short period of severe spasms and is able to walk off.
5 Severe muscle tremors following short movement (<10 m) or after slight disturbance. The animal collapses in severe tetanic spasms which may last in excess of 10 minutes, before the animal attempts to regain its footing.
From Keogh, R.G. 1973 Induction and prevention of ryegrass staggers in grazing sheep. New Zealand Journal of Experimental Agriculture 1: 55-57

TABLE 3: Effect of endophytes on mean ryegrass staggers scores
(0-5 ascending scale) at Lincoln 1999-2008
  Endophyte
  Standard AR37
Summer    
1999 3.5 2.3
2000 2.7 2.1
2001* 2.6 1.0
2002* 2.8 0.0
2003* 2.8 0.0
2004 3.8 3.8
2005 3.8 0.0
2006 1.2 2.4
2007* 2.0 0.0
2008* 3.0 1.4
Mean 2.8 1.3
Autumn    
1999 1.0 2.0
2000 3.8 1.8
2004 3.8 0.4
2005 3.8 1.9
2006 trial A 4.0 1.2
2006 trial B 4.0 0.6
Mean 3.4 1.3
* summer and autumn grazing combined