Rapid method for measuring complex carbohydrates in mammalian tissue
A rapid method for measuring complex carbohydrates, particularly glycogen, in mammalian tissue, and more particularly in non-living mammalian tissue.
The measurement of glycogen in mammalian tissue is relevant for such things as, for example, the ability to use the results of the measurement as a determination of the ultimate pH of meat. This is also a direct measure of many of the qualities of meat. Traditional methods are slow to process results, can be hazardous, inaccurate, inconsistent, time-consuming and some also use aggressive chemical reagents.
A rapid method of measuring the complex carbohydrates in mammalian tissue, comprising the steps of:
- Extracting a sample of tissue to be tested;
- Forming a homogenous slurry of the sample with an aqueous solution;
- Adding sufficient hydrolysing enzyme for ensuring complete hydrolysis of glycogen in the slurry; and
- Measuring the concentration of glucose in the slurry.