Dr. Goetz Laible

Senior Scientist

E: goetz.laible@agresearch.co.nz

Qualifications

Götz obtained a PhD in biochemistry from the Free University of Berlin, Germany in 1989 followed by post-doctoral training at the Salk Institute in San Diego, USA, and the Research Institute of Molecular Pathology in Vienna, Austria.

Research interests & activities

With the breakthrough in animal cloning and the prospect for the targeted genetic improvement of livestock, Götz joined AgResearch in 1997. Since then, his research has focused on the development of technologies to genetically improve livestock for agriculturally relevant traits and the engineering of dairy animals for the production of biopharmaceuticals in milk. Götz’s team has been the first to transfer the concept of improving the composition of milk from mouse models into a dairy animal and generated several transgenic cattle lines which produce milk with novel properties associated with potential nutritional and processing benefits. In addition, the team has produced several lines of transgenic cattle to demonstrate proof-of-concept for biopharming with the expression of large amounts of recombinant human proteins in milk which have potential therapeutic applications for the treatment of human diseases. More recently, the concept has been successfully extended into a second dairy species with the production of transgenic goats engineered for the expression of an anti-cancer antibody in milk. The project has since progressed towards the development of the goat-produced antibody into an effective human pharmaceutical.

With the advent of genome editing and its advanced technical capabilities, the research focus now encompasses the development and validation of ‘Next Generation Breeding’ approaches relying on the direct introgression of desirable natural mutation into elite dairy cattle. In addition, genome editing capabilities are applied for the development of large animal models to aid the investigation and therapy of human diseases and provide solutions for regenerative medicine.

Publications

Laible, G., Smolenski, G., Wheeler, T. and Brophy, B. (2016). Increased gene dosage for β- and κ-casein in transgenic cattle improves milk composition through complex effects. Scientific Reports 6, 37607. Full text link (http://rdcu.be/mTw3).

Tizard, M., Hallerman, E., Fahrenkrug, S., Newell-McGloughlin, M., Gibson, J., de Loos, F., Wagner, S., Laible, G., Han, J.Y., D\’Occio, M., Kelly, L., Lowenthal, J., Gobius, K., Silva, P., Cooper, C., Doran, T. (2016). Strategies to enable the adoption of animal biotechnology to sustainably improve global food safety and security. Transgenic Research 5, 575-595. Full text link (http://rdcu.be/n01n).

Bi, Y., Hua, Z., Liu, X., Hua, W., Ren, H., Xiao, H., Zhang, L., Li, L., Wang, Z., Laible, G., Wang, Y., Dong, F., Zheng, X. (2016). Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP. Scientific Reports 6, 31729. Full text link (http://rdcu.be/n01t).

Wagner, S., Thresher, R., Bland, R. and Laible, G. (2015). Adeno-associated-virus-mediated transduction of the mammary gland enables sustained production of recombinant proteins in milk. Scientific Reports 5, 15115. Full text link (http://rdcu.be/n01A).

Wei, J., Wagner, S., Lu, D., Maclean, P., Carlson, D.F., Fahrenkrug, S.C. and Laible, G. (2015). Efficient introgression of allelic variants by embryo-mediated editing of the bovine genome. Scientific Reports 5, 11735. Full text link (http://rdcu.be/n01D).

Laible, G., Wei, J. and Wagner, S. (2015). Improving livestock for agriculture – technological progress from random transgenesis to precision genome editing heralds a new era. Biotechnology Journal 10, 109-120. Full text link (https://www.ncbi.nlm.nih.gov/pubmed/25515661).

Al-Ghobashy, M.A., Williams, M.A.K., Laible, G. and Harding, D.R.K. (2013). Coupled solid phase extraction and microparticle-based stability and purity-indicating immunosensor for the determination of recombinant human myelin basic protein in transgenic milk. Talanta 109, 7-12. Full text link (https://www.ncbi.nlm.nih.gov/pubmed/23618134).

Antony, J., Oback, F., Chamley, L.W., Oback, B. and Laible, G. (2013). Transient JMJD2B-mediated reduction of H3K9me3 levels improves reprogramming of embryonic stem cells into cloned embryos. Molecular and Cellular Biology 33, 974-983. Full text link (http://mcb.asm.org/content/33/5/974.long).

Eghbalsaied, S., Ghaedi, K., Laible, G., Hoseini, S.M., Forouzanfar, M., Hajian, M., Oback, F., Nasr-Esfahani, M. and Oback, B. (2013). Exposure to DNA is insufficient for in vitro transgenesis of live bovine sperm and embryos. Reproduction 145, 97-108. Full text link (http://www.reproduction-online.org/content/145/1/97.long).

Jabed, A., Wagner, S., McCracken, J., Wells, D. N. and Laible, G. (2012). Targeted micro RNA expression in dairy cattle directs production of beta-lactoglobulin-free, high casein milk. Proceedings of the National Academy of Sciences of the United States of America 109, 16811-16816. Full text link (http://www.pnas.org/content/109/42/16811.long).

More related stories

Goats producing anti-cancer antibodies
http://www.newshub.co.nz/home/health/2016/03/goats-recruited-in-fight-against-cancer.html

Biotechnology Learning Hub – Transgenic cows
http://biotechlearn.org.nz/focus_stories/transgenic_cows

Current information on genome editing – Royal Society of New Zealand
http://www.royalsociety.org.nz/expert-advice/papers/yr2016/gene-editing/gene-editing-resources/