Dr. Karl Fraser

Senior Research Scientist

E: karl.fraser@agresearch.co.nz

Qualifications

BSc Massey, PhD Auckland

Research interests & activities

  • Plant and Mammalian Metabolomics
  • Mass Spectrometry & Chromatography
  • Analytical chemistry & method development
  • Food contamination/adulteration detection

Publications

Fraser, K., Harrison, S. J., Lane, G. A., Otter, D. E., Hemar, Y., Quek, S. Y., & Rasmussen, S. (2014). Analysis of low molecular weight metabolites in tea using mass spectrometry-based analytical methods. Critical Reviews in Food Science and Nutrition, 54(7), 924-937. doi: 10.1080/10408398.2011.619670     

Fraser, K., Lane, G. A., Otter, D. E., Harrison, S. J., Quek, S. Y., Hemar, Y., & Rasmussen, S. (2014). Non-targeted analysis by LC-MS of major metabolite changes during the oolong tea manufacturing in New Zealand. Food Chemistry, 151, 394-403. doi: 10.1016/j.foodchem.2013.11.054

Mackintosh, C. G., Cowie, C., Fraser, K., Johnstone, P., & Mason, P. C. (2014). Reduced efficacy of moxidectin and abamectin in young red deer (Cervus elaphus) after 20 years of moxidectin pour-on use on a New Zealand deer farm. Veterinary Parasitology, 199(1-2), 81-92. doi: 10.1016/j.vetpar.2013.09.028

Fraser, K., Lane, G. A., Otter, D. E., Harrison, S. J., Quek, S. Y., Hemar, Y., & Rasmussen, S. (2013). Monitoring tea fermentation/manufacturing by direct analysis in real time (DART) mass spectrometry. Food Chemistry, 141(3), 2060-2065. doi: 10.1016/j.foodchem.2013.05.054

Fraser, K., Lane, G. A., Otter, D. E., Hemar, Y., Quek, S. Y., Harrison, S. J., & Rasmussen, S. (2013). Analysis of metabolic markers of tea origin by UHPLC and high resolution mass spectrometry. Food Research International, 53(2), 827-835. doi: 10.1016/j.foodres.2012.10.015

Johnson, L. J., Koulman, A., Christensen, M., Lane, G. A., Fraser, K., Forester, N., Johnson, R. D., Bryan, G. T., & Rasmussen, S. (2013). An extracellular siderophore is required to maintain the mutualistic interaction of Epichloë festucae with Lolium perenne. PLoS Pathogens, 9(5), 1-18. doi: 10.1371/journal.ppat.1003332

Franzmayr, B. K., Rasmussen, S., Fraser, K. M., & Jameson, P. E. (2012). Expression and functional characterization of a white clover isoflavone synthase in tobacco. Annals of Botany, 110(6), 1291-1301. doi: 10.1093/aob/mcs168

Fraser, K., Harrison, S. J., Lane, G. A., Otter, D. E., Hemar, Y., Quek, S. Y., & Rasmussen, S. (2012). HPLC-MS/MS profiling of proanthocyanidins in teas: A comparative study. Journal of Food Composition and Analysis, 26(1-2), 43-51. doi: 10.1016/j.jfca.2012.01.004

Fraser, K., Harrison, S. J., Lane, G. A., Otter, D. E., Hemar, Y., Quek, S. Y., & Rasmussen, S. (2012). Non-targeted analysis of tea by hydrophilic interaction liquid chromatography and high resolution mass spectrometry. Food Chemistry, 134(3), 1616-1623. doi: 10.1016/j.foodchem.2012.03.045

Hancock, K. R., Collette, V., Fraser, K., Greig, M., Xue, H., Richardson, K., Jones, C., & Rasmussen, S. (2012). Expression of the R2R3-MYB transcription factor TaMYB14 from Trifolium arvense activates proanthocyanidin biosynthesis in the legumes Trifolium repens and Medicago sativa. Plant Physiology, 159(3), 1204-1220. doi: 10.1104/pp.112.195420

Harrison, S. J., Fraser, K., Lane, G. A., Villas-Boas, S. G., & Rasmussen, S. (2012). Automated high through-put analysis of fractions generated during the isolation of natural products. New Zealand Journal of Agricultural Research, 55(1), 15-20. doi: 10.1080/00288233.2011.629662

Koulman, A., Lee, T. V., Fraser, K., Johnson, L., Arcus, V., Lott, J. S., Rasmussen, S., & Lane, G. (2012). Identification of extracellular siderophores and a related peptide from the endophytic fungus Epichloë festucae in culture and endophyte-infected Lolium perenne. Phytochemistry, 75, 128-139. doi: 10.1016/j.phytochem.2011.11.020

Rasmussen, S., Lane, G. A., Mace, W., Parsons, A. J., Fraser, K., & Xue, H. (2012). The use of genomics and metabolomics methods to quantify fungal endosymbionts and alkaloids in grasses. Methods in Molecular Biology, 860, 213-226. doi: 10.1007/978-1-61779-594-7_14

Koulman, A., Lane, G. A., Christensen, M. J., Fraser, K., & Tapper, B. A. (2007). Peramine and other fungal alkaloids are exuded in the guttation fluid of endophyte-infected grasses. Phytochemistry, 68(3), 355-360. doi: 10.1016/j.phytochem.2006.10.012

Koulman, A., Tapper, B. A., Fraser, K., Cao, M., Lane, G. A., & Rasmussen, S. (2007). High-throughput direct-infusion ion trap mass spectrometry: A new method for metabolomics. Rapid Communications in Mass Spectrometry, 21(3), 421-428. doi: 10.1002/rcm.2854

Schreurs, N. M., McNabb, W. C., Tavendale, M. H., Lane, G. A., Barry, T. N., Cummings, T., Fraser, K., López-Villalobos, N., & Ramírez-Restrepo, C. A. (2007). Skatole and indole concentration and the odour of fat from lambs that had grazed perennial ryegrass/white clover pasture or Lotus corniculatus. Animal Feed Science and Technology, 138(3-4), 254-271. doi: 10.1016/j.anifeedsci.2006.11.020

Schreurs, N. M., Tavendale, M. H., Lane, G. A., Barry, T. N., McNabb, W. C., Cummings, T., Fraser, K., & López-Villalobos, N. (2007). The effect of supplementation of a white clover or perennial ryegrass diet with grape seed extract on indole and skatole metabolism and the sensory characteristics of lamb. Journal of the Science of Food and Agriculture, 87(6), 1030-1041. doi: 10.1002/jsfa.2802

Spencer, P., Sivakumaran, S., Fraser, K., Foo, L. Y., Lane, G. A., Edwards, P. J. B., & Meagher, L. P. (2007). Isolation and characterisation of procyanidins from Rumex obtusifolius. Phytochemical Analysis, 18(3), 193-203. doi: 10.1002/pca.967

Sivakumaran, S., Rumball, W., Lane, G. A., Fraser, K., Foo, L. Y., Yu, M., & Meagher, L. P. (2006). Variation of proanthocyanidins in Lotus species. Journal of Chemical Ecology, 32(8), 1797-1816. doi: 10.1007/s10886-006-9110-3

Young, O. A., Lane, G. A., Podmore, C., Fraser, K., Agnew, M. J., Cummings, T. L., & Cox, N. R. (2006). Changes in composition and quality characteristics of ovine meat and fat from castrates and rams aged to 2 years. New Zealand Journal of Agricultural Research, 49(4), 419-430

Tavendale, M. H., Lane, G. A., Schreurs, N. M., Fraser, K., & Meagher, L. P. (2005). The effects of condensed tannins from Dorycnium rectum on skatole and indole ruminal biogenesis for grazing sheep. Australian Journal of Agricultural Research, 56(12), 1331-1337. doi: 10.1071/ar04232

Meagher, L. P., Lane, G., Sivakumaran, S., Tavendale, M. H., & Fraser, K. (2004). Characterization of condensed tannins from Lotus species by thiolytic degradation and electrospray mass spectrometry. Animal Feed Science and Technology, 117(1-2), 151-163. doi: 10.1016/j.anifeedsci.2004.08.007

Roy, N. C., Fraser, K., Lane, G. A., Sinclair, B. R., & McNabb, W. C. (2004). Polyethylene glycol increases intestinal absorption and hepatic uptake of indole and skatole in sheep fed sulla. Journal of Animal and Feed Sciences, 13(Suppl. 1), 339-342

Sivakumaran, S., Meagher, L. P., Foo, L. Y., Lane, G. A., Fraser, K., & Rumball, W. (2004). Erratum: Floral Procyanidins of the Forage Legume Red Clover (Trifollum pratense L.) (Journal of Agricultural and Food Chemistry (2004) 52 (1581)). Journal of Agricultural and Food Chemistry, 52(9), 2740. doi: 10.1021/jf040137z

Sivakumaran, S., Meagher, L. P., Foo, L. Y., Lane, G. A., Fraser, K., & Rumball, W. (2004). Floral procyanidins of the forage legume red clover (Trifolium pratense L.). Journal of Agricultural and Food Chemistry, 52(6), 1581-1585. doi: 10.1021/jf035379y

Sivakumaran, S., Molan, A. L., Meagher, L. P., Kolb, B., Foo, L. Y., Lane, G. A., Attwood, G. A., Fraser, K., & Tavendale, M. (2004). Variation in antimicrobial action of proanthocyanidins from Dorycnium rectum against rumen bacteria. Phytochemistry, 65(17), 2485-2497. doi: 10.1016/j.phytochem.2004.08.046

Panaccione, D. G., Tapper, B. A., Lane, G. A., Davies, E., & Fraser, K. (2003). Biochemical outcome of blocking the ergot alkaloid pathway of a grass endophyte. Journal of Agricultural and Food Chemistry, 51(22), 6429-6437. doi: 10.1021/jf0346859

Young, O. A., Lane, G. A., Priolo, A., & Fraser, K. (2003). Pastoral and species flavour in lambs raised on pasture, lucerne or maize. Journal of the Science of Food and Agriculture, 83(2), 93-104. doi: 10.1002/jsfa.1282

Lane, G. A., & Fraser, K. (1999). A comparison of phenol and indole flavour compounds in fat, and of phenols in urine of cattle fed pasture or grain. New Zealand Journal of Agricultural Research, 42(3), 289-296