Dr. Linley Schofield

Senior Rumen Molecular Biologist/Biochemist

E: Linley.Schofield@agresearch.co.nz

Qualifications

BSc MSc DPhil Waikato

Research interests & activities

Enzyme structure and function.
Methanogen enzymes; expression, purification, characterisation and structural determination.
Enzyme assay development and high-throughput screening.
Discovery of small molecule inhibitors for methane mitigation.

Professional Memberships

  • New Zealand Microbiological Society
  • New Zealand Institute of Chemistry

Publications

Carbone, V., Schofield, L.R., Zhang, Y., Sang, C., Dey, D., Hannus, I.M., Martin, W.F., Sutherland-Smith, A.J., Ronimus, R.S. (2015). Structure and evolution of the archaeal lipid synthesis enzyme sn-glycerol-1-phosphate dehydrogenase. Journal of Biological Chemistry, 290, 21690-21704.
 
Schofield, L.R., Beattie, A.K., Tootill, C.M., Dey, D., Ronimus, R.S. (2015). Biochemical characterisation of phage pseudomurein endoisopeptidases PeiW and PeiP using synthetic peptides. Archaea 2015: 828693.
 
Nazmi, A.R., Schofield, L.R., Dobson, R.C.J., Jameson, G.B., Parker, E.J. (2014). Destabilization of the homotetrameric assembly of 3-deoxy-D-arabino heptulosonate 7-phosphate synthase from the hyperthermophile Pyrococcus furiosus enhances enzymatic activity. Journal of Molecular Biology, 426, 656-673.
 
Carbone, V., Schofield, L.R., Beattie, A.K., Sutherland-Smith, A.J., Ronimus, R.S. (2013). The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from Methanobrevibacter ruminantium. Proteins, 81, 2064-70.

Mittelstädt, G., Negron, L., Schofield, L.R., Marsh, K., Parker, E.J. (2013). Biochemical and structural characterisation of dehydroquinate synthase from the New Zealand kiwifruit Actinidia chinensis. Archives of Biochemistry and Biophysics, 537, 185-91.
 
Tran, D., Pietersma, A.L., Schofield, L.R., Rost, M., Jameson, G.B., Parker, E.J. (2011). Investigating the role of the hydroxyl groups of substrate erythrose 4-phosphate in the reaction catalysed by the first enzyme of the shikimate pathway. Bioorganic and Medicinal Chemistry Letters, 21, 6838-6841.Ahn, M., Pietersma, A.L., Schofield, L.R., Parker, E.J. (2005). Mechanistic divergence of two closely related aldol-like enzyme-catalysed reactions. Organic and Biomolecular Chemistry, 3, 4046-4049.